两株芽孢杆菌混菌发酵产芽孢条件的优化%Optimization of spore-producing conditions of two Bacillus spp.mixed fermentation
河南省科学院生物研究所有限责任公司,河南郑州450008;河南省微生物工程重点实验室,河南郑州450008 河南省科学院生物研究所有限责任公司,河南郑州,450008
2017-05-01
地衣芽孢杆菌混菌发酵DY-1 胶冻样芽孢杆菌XK 产芽孢条件 正交试验
为了探索地衣芽孢杆菌(Baclicus lincheniformis) DY-1和胶冻样芽孢杆菌(Bacillus mucilaginosus) XK混菌发酵产芽孢的最佳条件,该研究采用单因素试验和正交试验进行了优化;利用Eppendorf 7.5 L发酵罐在最佳条件下进行了混菌发酵产芽孢试验.结果表明,混菌发酵产芽孢的最佳发酵条件为:胶冻样芽孢杆菌XK和地衣芽孢杆菌DY-1种子液的接种比例为4:1(V/V),培养温度37℃,培养时间60h,pH 8.0.在此条件下,进行Eppendorf 7.5L发酵罐混菌发酵试验,其芽孢数可高达1.1x1011 CFU/mL,高于摇瓶发酵所产芽孢数(2.0× 1010 CFU/mL).%In order to explore the optimum spore-producing conditions of Bacillus licheniformis DY-1 and Bacillus mucilaginosus XK mixed fermentation,the study was optimized by single factor and orthogonal experiments.Eppendorf 7.5 L fermentor was used to produce spores under the optimum conditions.The results showed that the optimum spore-producing conditions of two Bacillus spp.mixed fermentation were as follows:the seed solution inoculation proportion ofB.mucilaginosus XK 4:1 and B.licheniformis DY-1 (V/V),culture temperature 37 ℃,culture time 60 h and pH 8.0.Under the conditions,the spore count was up to 1.1 × 1011 CFU/ml in Eppendorf 7.5 L fermentor,which was higher than that of shaking flask fermentation (2.0× 1010 CFU/ml).
