克鲁维酵母(K.hubeiensis)产菊粉酶的发酵参数研究%A Priliminary Study on Inulinase Fermentation Factors of Kluyvreromyces hubeiensis Strain
河南省微生物工程重点实验室,郑州,450008;河南省科学院生物研究所有限责任公司,郑州,450008 河南省微生物工程重点实验室,郑州,450008 河南农业大学,郑州,450002 河南省科学院生物研究所有限责任公司,郑州,450008
2011-04-27
菊粉酶 湖北克鲁维酵母(K.hubeiensis) 3,5-二硝基水杨酸法
菊粉酶(inulinase)是一种降解菊糖B-2,1-D-果聚糖果糖苷键生成果糖或低聚果糖的水解酶.本研究在筛选菊粉酶高产菌株基础上,对产酶能力较强的l株湖北克鲁维酵母(Kluyvreromyces hubeiensis)的发酵产酶参数进行了优化;结果表明,该菌株在菊糖3%,蛋白胨3%,pH 5.0,30~37℃摇床培养48 h的产酶活力最高,其单位酶活比条件优化前提高了8~10倍.%Inulin is a natural fructan,a plant storage carbohydrate in the body.Inulinase is the hydrolysis of inulin to β-2,1-D-glycosidic bonds fruit candy together a class of enzymes.With a lot of wild fungus strains for type,screens after DNS process to one produces the inulase high strain,and has conducted the fermentation condition research to it,in the inulin 3 %,protein peptone 3 %,pH 5.0,the culture temperature in 30~37 ℃ about,the table raises 48~50 h to obtain the enzyme activity to be as higher as 8~10 times than the preliminary strain.
