克服香石竹组织培养中玻璃苗的研究%A STUDY ON DECREASE IN VITRIFICATION SHOOTS OF CARNATION (DIANTHUS CARYOPHYLLUS L. ) IN TISSUE CULTURE
河南省科学院生物研究所,河南,郑州,450008 郑州中多电子计算机网络公司,河南,郑州,450000 河南省中医院,河南,郑州,450003
2005-10-11
香石竹 组织培养 玻璃苗 玻璃化现象
香石竹试管苗玻璃化现象给试管苗的应用带来困难,采用强光照10000-20000 LX,在培养基中提高蔗糖和琼脂浓度,降低激素用量,对克服香石竹试管苗玻璃化有明显效果.青霉素对克服香石竹试管苗玻璃化无明显效果,但对生根有促进作用,其作用机制有待进一步研究.%The phenomenon of Vitrification phenomenon is a big obstruction in the propagantion of carnation by tissue culture, the effective way to decrease the vitrification phenomenon is to increase the concentration of sucrose and agar in the medium, to use strong luminosity of 10000-20000 LX, and to decrease the concentration of hormone. There is not evident effect of penicillin to control the vitrification shoots, but penicillin can stimulate the growth of carnation plantlet root in the medium, the mechanism of penicillin function to the root is not clear and it wll be studied further.
