滚环DNA扩增的原理、应用和展望%PRINCIPLE AND APPLICATION OF ROLLING CIRCLE DNA AMPLIFICATION
河南省科学院,郑州,450003 国家药品生物制品检定所,北京,100050 河南康鑫生物技术有限公司,郑州,450003
2004-09-19
滚环DNA扩增 PHI29DNA聚合酶 微阵列 痕量检测
滚环DNA扩增(ROLLING CIRCLE DNA AMPLIFICATION,RCA)是一种等温信号扩增方法,其线性扩增倍数为105,指数化扩增能力大于109,产生的扩增产物连接在固相支持物(如玻片、微孔板等)表面的DNA引物或抗体上.RCA是一种适合在芯片上(ON-CHIP)进行信号扩增的新技术,它既能提供研究分析的敏感性和特异性,又能保持立体分析的多元性.RCA亦是一种痕量的分子检测方法,可用于极其微量的生物大分子和生物标志的检测与研究.%Rolling circle DNA amplification (RCA) is an isothermal signal amplification method.Phi 29 DNA polymerase can synthesize DNA strands>70kbp in length and achieve a 10 5-fold linear and a 10 8-fold or more exponential amplification.The product produced by RAC can be linked to the primers or antibodies on the surface of solid phase(such as glass,microplate etc.) that make the RCA especially suitable for biochip research.As a new technique RCA is well suited to on-chip signal amplification that not only provide the sensibility and specificity of analysis but also keep the multiplexing of stereo-analysis.RCA is also a trace molecular detection method and will be exploited in research and detection of extremely trace biomacromolecule or biomarkers.;
